Journal: PLOS One

Article Title: Matrix metalloproteinase-7 is dispensable in a mouse model of sepsis-induced acute lung injury

doi: 10.1371/journal.pone.0321349

Figure Lengend Snippet: Septic WT mice had significantly higher peritoneal wash inflammatory cell counts (A), peritoneal wash neutrophil counts (B), and plasma concentrations of concentrations of TNF-α (C), IL-1β (D), IL-6 (E), CXCL-1 (F), IL-12p70 (G), IFN-γ (H), and IL-10 (I) compared to their control mice. Similar significant increases were observed for septic MMP7KO mice compared to their controls, except for plasma IL-12p70 (G), IFN-γ(H). In septic MMP7KO mice plasma IL-10 (I; p =0.0811) trended towards an increased concentration compared to MMP7KO control mice. No differences were observed between MMP7KO and WT mice in either treatment group. N = 6-19. [Statistical analysis: Kruskal-Wallis test with a Dunn’s multiple comparisons test]. Each point represents an individual male (solid circle) or female (open circle) animal. Horizontal line indicates combined median of male and female animals. Control = 5% dextrose + room air at 21% O 2 . TNF-α = tumor necrosis factor-α, IL-12p70 = interleukin-12p70, IL-6 = interleukin-6, IL-1β = interleukin-1β, and CXCL-1 = C-X-C motif chemokine ligand 1, IFN-γ = interferon gamma, IL-10 = interleukin-10.

Article Snippet: MMP2 (Mm00439498_m1), MMP9 (Mm0042991_m1), IL-6 (Mm00446190_m1), IL-1β(Mm00434228_m1), IFN-γ (Mm01168134_m1), and CXCL-1(Mm04207460_m1) expression were also normalized to GAPDH (Mm99999915_g1) expression but using Taqman PrimePCR probes (ThermoFisher Scientific, Rockford, IL).

Techniques: Clinical Proteomics, Control, Concentration Assay

Journal: PLOS One

Article Title: Matrix metalloproteinase-7 is dispensable in a mouse model of sepsis-induced acute lung injury

doi: 10.1371/journal.pone.0321349

Figure Lengend Snippet: Septic mice (in both WT and MMP7KO) had significantly higher BAL total cell counts (A), and BAL concentrations of TNF-α (B), IL-1β (C), and CXCL-1 (D), and IL-10 (E), as well as lung tissue IL-1β (F) and CXCL-1 (G) mRNA expression compared to their control mice. Septic WT mice also had significantly higher BAL concentrations of IL-6 (H), as well as lung tissue IFN-γ (I) and IL-6 mRNA (J) compared to WT controls, with BAL IL-12p70 (K) being numerical higher. Septic MMP7KO mice had numerically higher BAL IL-6 concentrations as well a lung tissue CXCL-1 and IL-6 mRNA compared to MMP7KO control mice, but did not differ with regards to lung tissue IFN-γmRNA expression or BAL concentrations of IL-12p70. Septic mice did not differ from control mice across either genotype for BAL IFN-γ (L) or BAL neutrophils (M). No differences between MMP7KO and WT genotypes were observed for any outcomes of lung inflammation in either treatment group. N = 6-19. [Statistical analysis: Kruskal-Wallis test with a Dunn’s multiple comparisons test]. Each point represents an individual male (solid circle) or female (open circle) animal. Horizontal line indicates combined median of male and female animals. Control = 5% dextrose + room air at 21% O 2 , BAL= bronchoalveolar lavage, TNF-α = tumor necrosis factor-α, IL-12p70 = interleukin-12p70, IL-6 = interleukin-6, IL-1β = interleukin-1β, and CXCL-1 = C-X-C motif chemokine ligand 1, IFN-γ = interferon gamma, IL-10 = interleukin-10.

Article Snippet: MMP2 (Mm00439498_m1), MMP9 (Mm0042991_m1), IL-6 (Mm00446190_m1), IL-1β(Mm00434228_m1), IFN-γ (Mm01168134_m1), and CXCL-1(Mm04207460_m1) expression were also normalized to GAPDH (Mm99999915_g1) expression but using Taqman PrimePCR probes (ThermoFisher Scientific, Rockford, IL).

Techniques: Expressing, Control

Journal: PLOS One

Article Title: Matrix metalloproteinase-7 is dispensable in a mouse model of sepsis-induced acute lung injury

doi: 10.1371/journal.pone.0321349

Figure Lengend Snippet: For both genotypes, septic mice showed significantly higher plasma urea nitrogen concentrations (A), kidney tissue mRNA expression of IL-1β (B), IL-6 (C), CXCL-1 (D), and NGAL (D), as well as kidney tissue NGAL protein (F) compared to control mice. Septic WT mice also had significantly higher kidney tissue mRNA expression of KIM-1, while septic MMP7KO had numerically higher KIM-1 mRNA (H). However, septic mice did not differ from control for kidney tissue KIM-1 protein in either genotype (G). No differences were observed between WT and MMP7KO mice in either treatment group. Representative western blots are displayed for NGAL (E) and KIM-1 (H) relative to β-actin. N = 6-20. [Statistical analysis: Kruskal-Wallis test with a Dunn’s multiple comparisons test]. Each point represents an individual male (solid circle) or female (open circle) animal. Horizontal line indicates combined median of male and female animals. Control = 5% dextrose + room air at 21% O 2 . IL-1β = interleukin-1β, IL-6 = interleukin-6, and CXCL-1 = C-X-C motif chemokine ligand 1, NGAL = neutrophil gelatinase-associated lipocalin, KIM-1 = kidney injury marker-1.

Article Snippet: MMP2 (Mm00439498_m1), MMP9 (Mm0042991_m1), IL-6 (Mm00446190_m1), IL-1β(Mm00434228_m1), IFN-γ (Mm01168134_m1), and CXCL-1(Mm04207460_m1) expression were also normalized to GAPDH (Mm99999915_g1) expression but using Taqman PrimePCR probes (ThermoFisher Scientific, Rockford, IL).

Techniques: Clinical Proteomics, Expressing, Control, Western Blot, Marker